You are currently viewing an outdated version of
SubtiWiki.
Please use
the newest version!
yrrO [2020-07-01 09:56:12]
tRNA uridine hydroxylase (in complex with
YrrN)
Molecular weight
47.46 kDa
Function
introduction of 5-methoxyuridine modification in tRNA (U34)
Product
tRNA uridine hydroxylase
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
2,792,860 → 2,794,128
Phenotypes of a mutant
50% reduction in mo5U tRNA levels PubMed The protein
Catalyzed reaction/ biological activity
hydroxylation of U34 in tRNA to give 5-hydroxyuridine (ho5U) (in complex with YrrN) with prephenate as hydroxyl donor, ho5U is the precursor for 5-methoxyuridine modification (by TrmR) PubMed Protein family
peptidase U32 family (with YrrN, according to UniProt) Paralogous protein(s)
Expression and Regulation
Biological materials
Mutant
MGNA-A007 (yrrO::erm), available at the NBRP B. subtilis, JapanBKE27340 (ΔyrrO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGCTTAACCTCCTTTTG, downstream forward: _UP4_ATGAGAAAGGGGAAGTAACABKK27340 (ΔyrrO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGCTTAACCTCCTTTTG, downstream forward: _UP4_ATGAGAAAGGGGAAGTAACA References