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yrrO [2020-07-01 09:56:12]
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yrrO [2020-07-01 09:56:12]

tRNA uridine hydroxylase (in complex with YrrN)
Locus
BSU_27340
Isoelectric point
4.85
Molecular weight
47.46 kDa
Protein length
422 aa Sequence Blast
Gene length
1269 bp Sequence Blast
Function
introduction of 5-methoxyuridine modification in tRNA (U34)
Product
tRNA uridine hydroxylase
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

    • Gene

    Coordinates
    2,792,860 → 2,794,128

    Phenotypes of a mutant

  • 50% reduction in mo5U tRNA levels PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • hydroxylation of U34 in tRNA to give 5-hydroxyuridine (ho5U) (in complex with YrrN) with prephenate as hydroxyl donor, ho5U is the precursor for 5-methoxyuridine modification (by TrmR) PubMed

    • Protein family

  • peptidase U32 family (with YrrN, according to UniProt)

    • Paralogous protein(s)

  • YrrN

    • Expression and Regulation

    Operons

    Genes
    trmR-yrrN-yrrO-udk-greA
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-A007 (yrrO::erm), available at the NBRP B. subtilis, Japan
  • BKE27340 (ΔyrrO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGCTTAACCTCCTTTTG, downstream forward: _UP4_ATGAGAAAGGGGAAGTAACA
  • BKK27340 (ΔyrrO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGCTTAACCTCCTTTTG, downstream forward: _UP4_ATGAGAAAGGGGAAGTAACA

    • References

    Research papers

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